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Tissue testing used to assess the chemical contents in potato plants is considered laborious, time-consuming, destructive, and expensive. Ground-based sensors have been assessed to provide efficient information on nitrogen using leaf canopy reflectance. In potatoes, however, the main organ required for tissue testing is the petiole to estimate the elements of all nutrients. This research aims to assess whether there is a correlation between the chemical contents of potato petioles and leaf spectrum, and to examine whether the spectrum of dried or fresh leaves have higher correlation values. Petiole chemical contents of all elements were tested as a reference point. Leaves were split equally into dried and fresh groups for spectral analysis (400–2500 nm). Lasso Regression models were built to estimate concentrations in comparison to actual values. The performances of the model were tested using the Ratio of …