查看文章

Clustered regularly interspaced short palindromic sequences (CRISPR)-Cas is one of the most promising gene editing systems. Defined by its specificity, sensitivity, and high base resolution for nucleic acid recognition, CRISPR-Cas has emerged as a tool of choice for biosensing. Biosensing via CRISPR-Cas utilizes the trans-cleavage activity of effectors on various nano-platforms so that different targets, such as viral and bacterial infections, genetic mutations, and tumor biomarkers, could be detected. Various sensing methods employed in these sensors include fluorescence, colorimetric, luminescence resonance, and electrochemical readouts. CRISPR-Cas biosensing offers multiple possibilities for multiplex diagnosis of different diseases by detecting many analytes from one specimen. In this review, we have explored recent advances in CRISPR-based biosensors. Due to their high accuracy and versatility …