作者
Shahriar Alipour, Ebrahim Sakhinia, Alireza Khabbazi, Nasser Samadi, Zohreh Babaloo, Mahdi Azad, Somayeh Abolhasani, Jafar Farhadi, Golamreza Jadideslam, Neda Roshanravan, Mohammad Nouri
发表日期
2020/5/1
期刊
Reumatologia clinica
卷号
16
期号
3
页码范围
229-234
出版商
Elsevier Doyma
简介
Background
IL-6 mRNA expression is significantly high in many autoimmune diseases such as Behçet's disease; this is often related with more aggressive phenotypes. Nevertheless, the essential molecular process for its high expression has not been completely realized. The aim of this study was undertaken to estimate the gene copy number variation and promoter methylation to IL-6's high expression.
Methods
This study was performed on 51 patients and 61 healthy controls. Initially, DNA and RNA were extracted from all specimens. Promoter methylation levels of IL-6 were evaluated by MeDIP-qPCR technique. Also, IL-6 gene expression was measured by Real-time PCR. After that, we evaluated the relationship between gene expression and methylation, as well as their relationship with clinical specification.
Results
As we expected, the expression level of IL-6 gene increased significantly in the patient group …
学术搜索中的文章
S Alipour, E Sakhinia, A Khabbazi, N Samadi… - Reumatologia clinica, 2020