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Background: Fatty acid metabolites play a key role in numerous physiological and pathological processes. A rapid liquid chromatography-mass spectrometry assay for the simultaneous determination of prostanoids, isoprostane and lipoxygenase (LOX) derived fatty acid metabolites in a small biological sample of only 20 μL was developed.

Methods: Human plasma samples were applied to a filter spot, extracted without prior derivatization and analyzed within 13 min. Detection of metabolites was performed on a triple quadrupole mass spectrometer in negative multiple-reaction monitoring detection mode. Application of this assay to various biological matrices was performed.

Results: The validated assay was linear over the concentration range of 5–500 nmol/L for prostanoids and isoprostane, 50–5000 nmol/L for LOX-derived metabolites and 400–40,000 nmol/L for fatty acids. Limits of quantitation were 0.4–233 …