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Lactobacillus acidophilus FTCC 0291,Lactobacillus bulgaricus FTCC 0411Lactobacillus casei FTCC 0442,Lactobacillus fermentum FTD 13 andBifidobacterium bifidum BB 12 were screened for their α-galactosidase activity over 24 h.Lactobacillus bulgaricus FTCC 0411 andL. fermentum FTD 13 showed highest α-galactosidase specific activity and were selected to be incorporated into tofufa for a storage study of 9 days at 25 and 4 °C. The viability of both probiotics in tofufa exceeded 10⁶ CFU/g and was maintained over storage, mainly contributed by their ability to hydrolyse oligosaccharides and to utilise the reducing sugars produced. The presence of probiotics in tofufa showed an increase in the concentrations of organic acids which led to a decrease in pH levels. This exhibited a preservative effect, where total aerobes and total anaerobes were 2 log₁₀ CFU/g lower than the control. Probiotics in tofufa …