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The full height rectangular opening castellated steel beam failed in Vierendeel mechanism. This makes the flexural capacity of castellated steel beam is lower than the original IWF section. This paper discusses analysis and test result of a full height rectangular opening castellated steel beam with diagonal stiffener. The diagonal stiffener used in this research has a purpose of preventing Vierendeel mechanism. This research used two specimens, a short span specimen to study shear behavior, and a long span specimen to study flexural behavior. Test results show that the long span specimen can avoid Vierendeel mechanism and increase the yield moment capacity by 1.6 times of the original IWF section. The failure of the short span specimen is a combination of shear failure and Vierendeel mechanism as in elastic condition, the diagonal stiffener, flange, and web post were worked and failed together. Based on …