作者
Sebastian Niedenführ, Wolfgang Wiechert, Katharina Nöh
发表日期
2015/8/1
来源
Current opinion in biotechnology
卷号
34
页码范围
82-90
出版商
Elsevier Current Trends
简介
Highlights
- Tracer experiments, analytics and modeling constitute the 13 C fluxomics triumvirate.
- 13 C fluxomics tools differ in complexity, information power and universality.
- The biological question prescribes the choice of the modeling approach.
- In turn, modeling poses unique challenges for experimental and analytical setups.
- Standards will raise throughput and flexibility of 13 C fluxomics.
Metabolic reaction rates (fluxes) contribute fundamentally to our understanding of metabolic phenotypes and mechanisms of cellular regulation. Stable isotope-based fluxomics integrates experimental data with biochemical networks and mathematical modeling to ‘measure’the in vivo fluxes within an organism that are not directly observable. In recent years, 13 C fluxomics has evolved into a technology with great experimental, analytical, and mathematical diversity. This review aims at establishing a unified taxonomy by means of …
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S Niedenführ, W Wiechert, K Nöh - Current opinion in biotechnology, 2015