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Although cycling is increasing in developed regions, such as Europe and North America, high-income developing countries in the Arabian Gulf region still have low cycling activities. Limited research has investigated the barriers to cycling in these countries. In this study, the barriers and motivators in Qatar, a high-income developing country, were investigated. Respondents were asked to report their bicycle usage during the last 12 months. The results indicated that approximately 15% used a bicycle during this period, but only 1.7% bicycled for transportation purposes. The analysis revealed the different barriers to cycling and their relative strengths. The study also compared the perceived challenges of cycling between males and females. The questionnaire results indicated that both groups considered the issues related to the weather conditions, bicycle ownership, lack of paths or connections, and driver behavior as important barriers to cycling. However, the female participants identified clothing, parental consent, and cultural and societal pressure as far more important. When asked about motivators for cycling, the results revealed that improving intersections, adding additional infrastructure facilities, planting trees for shading, affordable bicycles, and campaigns targeting potential cyclists and drivers are needed in order to increase cycling. To better understand how bike-sharing will be perceived if implemented in the future, the feedback was obtained from the participants, and their opinions indicated that there is a great deal of acceptance for this type of service. The outcome of this study can be of benefit to public agencies in developing …