作者
Susan K Murphy, Nilda Itchon-Ramos, Zachary Visco, Zhiqing Huang, Carole Grenier, Rose Schrott, Kelly Acharya, Marie-Helene Boudreau, Thomas M Price, Douglas J Raburn, David L Corcoran, Joseph E Lucas, John T Mitchell, F Joseph McClernon, Marty Cauley, Brandon J Hall, Edward D Levin, Scott H Kollins
发表日期
2018/12/2
期刊
Epigenetics
卷号
13
期号
12
页码范围
1208-1221
出版商
Taylor & Francis
简介
Little is known about the reproductive effects of paternal cannabis exposure. We evaluated associations between cannabis or tetrahydrocannabinol (THC) exposure and altered DNA methylation in sperm from humans and rats, respectively. DNA methylation, measured by reduced representation bisulfite sequencing, differed in the sperm of human users from non-users by at least 10% at 3,979 CpG sites. Pathway analyses indicated Hippo Signaling and Pathways in Cancer as enriched with altered genes (Bonferroni p < 0.02). These same two pathways were also enriched with genes having altered methylation in sperm from THC-exposed versus vehicle-exposed rats (p < 0.01). Data validity is supported by significant correlations between THC exposure levels in humans and methylation for 177 genes, and substantial overlap in THC target genes in rat sperm (this study) and genes previously reported as having …
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SK Murphy, N Itchon-Ramos, Z Visco, Z Huang… - Epigenetics, 2018