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Information and its security have attracted the research community in recent years with increasing usage of mobile applications. Mobile devices have different security options in data transmission such as reading some biometric values. The keystone of the modern block and stream ciphers is the use of a substitution box (s-box) that obscures correlation between plaintext and ciphertext. In this study, we proposed a novel s-box generation algorithm by using the fingerprint pattern of the person who transfers information to the target. We generated several s-boxes by using bifurcation and ridge ending features of the fingerprint. Proposed s-boxes are compared with several known s-boxes over nonlinearity, bijectiveness, strict avalanche criterion, bit independence criterion, linear probability, and differential probability. Along with these properties, we analyzed confidence interval and randomness properties of new s …