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A knowledge audit is a process whose purpose is to diagnose the situation in which knowledge is kept within an organization, that is, it allows to know what knowledge exists, who has it, where it is, how it moves and how it is managed. The aim of this paper is to present the implementation of an ontology as a support to knowledge audit process through a computer prototype. A case study in an organization is developed, in which the knowledge audit process is supported by the prototype. Among the advantages it offers: facilitates obtaining knowledge inventory, classifying and valuing knowledge assets and flows, identifying knowledge gaps, knowledge management analysis and reusing the audit's results. Copyright © 2009 John Wiley & Sons, Ltd.