查看文章

S‐acylation is an important lipid modification that primarily involves DHHC proteins (DHHCs) and associated S‐acylated proteins. No DHHC–S‐acylated protein pair has been reported so far in rice (Oryza sativa L.) and the molecular mechanisms underlying S‐acylation in plants are largely unknown. We constructed an OsDHHC cDNA library for screening corresponding pairs of DHHCs and S‐acylated proteins using bimolecular fluorescence complementation assays. Five DHHC–S‐acylated protein pairs (OsDHHC30–OsCBL2, OsDHHC30–OsCBL3, OsDHHC18–OsNOA1, OsDHHC13–OsNAC9, and OsDHHC14–GSD1) were identified in rice. Among the pairs, OsCBL2 and OsCBL3 were S‐acylated by OsDHHC30 in yeast and rice. The localization of OsCBL2 and OsCBL3 in the endomembrane depended on S‐acylation mediated by OsDHHC30. Meanwhile, all four OsDHHCs screened complemented the …