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Purpose:: Recent work by our group has demonstrated that using intra ocular illumination of the fundus can simplify the light paths associated with retinal imaging. Our group has also recently reported the use of a novel method to measure oxyhemoglobin saturation that is insensitive to changes in pH, path length or concentration and is more accurate than previous methods. In this abstract we report the use of these methods to measure oxygen saturation in the retinal arteries of swine during progressive hypoxia.

Methods:: Five swine retinae were illuminated using intraocular placement of a fiberoptic light pipe that diffusely places a spot of light on the retina at an oblique angle removing the vessel apex glint. Multiple wavelengths of light were introduced through the light pipe and the image of the retina obtained through the cornea, lens and pupil using a CCD Camera. The spectra from 460 nm to 521 nm were …