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Jujube shell (JS) is agricultural waste and environmentally friendly biosorbent was used for the removal of Congo red (CR) from aqueous solution in a fixed-bed column system. The biosorbent was characterized by FTIR, SEM and TGA-DTA analysis. Variables in the process were bed depths (2, 4 and 6 cm), flow rate (2.8, 4.5 and 6.4 min/L), influent CR concentrations (100, 200 and 300 mg/L) and particles size (50–100, 100–315, 315–500 and 500–1000 μm). The highest biosorption capacity (80.49 mg/g) on a 100 mg/L of CR solution was achieved within a flow rate of 2.8 mL/min, bed depth of 4 cm and JS particles size of 50–100 μm. The results of this study have shown that CR can be effectively adsorbed on JS. The breakthrough and exhaustion times decreased with increasing flow rate, decreasing bed depth, increasing influent CR concentration and increasing size particles. Thomas and Bohart-Adams models …