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For the first time, molecular information deduced from Raman spectroscopy measurements was spatially correlated with histopathologic analysis to detect and characterize prostate cancer (PCa) using Raman spectroscopy (RS).

Ex vivo post-prostatectomy specimen were interrogated using a handheld RS probe. Up to 50 areas (of 500µm in diameter each) per prostate slice were correlated spatially with hematoxylin, phloxine, and saffron (HPS) stained images for detailed histopathologic characteristics, including percentage of benign glands and cancer grade group (International Society of Urological Pathology, ISUP): (1) each measured area was identified with a spot of Indian ink; (2) a photograph was taken at the end of the measurements to build a mask including the prostate slice contour and circles corresponding to the measured areas; (3) the whole prostate slice was reconstructed from the HPS scans guided …