作者
Akiyoshi Hirayama, Kenjiro Kami, Masahiro Sugimoto, Maki Sugawara, Naoko Toki, Hiroko Onozuka, Taira Kinoshita, Norio Saito, Atsushi Ochiai, Masaru Tomita, Hiroyasu Esumi, Tomoyoshi Soga
发表日期
2009/6/1
期刊
Cancer research
卷号
69
期号
11
页码范围
4918-4925
出版商
American Association for Cancer Research
简介
Most cancer cells predominantly produce energy by glycolysis rather than oxidative phosphorylation via the tricarboxylic acid (TCA) cycle, even in the presence of an adequate oxygen supply (Warburg effect). However, little has been reported regarding the direct measurements of global metabolites in clinical tumor tissues. Here, we applied capillary electrophoresis time-of-flight mass spectrometry, which enables comprehensive and quantitative analysis of charged metabolites, to simultaneously measure their levels in tumor and grossly normal tissues obtained from 16 colon and 12 stomach cancer patients. Quantification of 94 metabolites in colon and 95 metabolites in stomach involved in glycolysis, the pentose phosphate pathway, the TCA and urea cycles, and amino acid and nucleotide metabolisms resulted in the identification of several cancer-specific metabolic traits. Extremely low glucose and high …
引用总数
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A Hirayama, K Kami, M Sugimoto, M Sugawara, N Toki… - Cancer research, 2009