作者
Florencia Payaslian, Victoria Gradaschi, Liliana Rondón Salazar, Maria Eugenia Dieterle, Estefanía Urdániz, Matias Di Paola, José Peña Cárcamo, Fabio Zon, Ezequiel Sosa, Darío Fernandez Do Porto, Matthew Dunne, Pauline Goeller, Jochen Klumpp, Raúl Ricardo Raya, Alejandro Reyes, Mariana Piuri
发表日期
2021/3/1
期刊
Therapy, Applications, and Research
卷号
2
期号
1
页码范围
43-49
出版商
Mary Ann Liebert, Inc., publishers
简介
Introduction: Because of the clinical relevance of Mycobacteria, and from a therapeutic perspective, there is an increasing interest to study phages that infect bacteria belonging to this genus.
Materials and Methods: A phage was isolated from a soil sample, using Mycobacterium smegmatis as host. Its characterization included sequencing, annotation, and analysis of the genome, host range determination, and electron microscopy imaging.
Results: Mycobacterium phage vB_MsmS_Celfi is a temperate phage able to infect Mycobacterium tuberculosis with high efficiency. From electron microscopy images, Celfi belongs to the Siphoviridae family. Genome analysis classified phage Celfi into cluster L, subcluster L2 of Actinobacteriophage clusters. Mycobacterium phage Celfi exhibits a Lysin B distant to those present in other members of the subcluster and other mycobacteriophages.
Conclusions: The discovery of new …
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F Payaslian, V Gradaschi, L Rondón Salazar… - Therapy, Applications, and Research, 2021