作者
Anna Z Wec, Daniel Wrapp, Andrew S Herbert, Daniel Maurer, Denise Haslwanter, Mrunal Sakharkar, Rohit K Jangra, M Eugenia Dieterle, Asparouh Lilov, Deli Huang, V Tse Longping, Nicole V Johnson, Ching-Lin Hsieh, Nianshuang Wang, Juergen H Nett, Elizabeth Champney, Irina Burnina, Michael Brown, Shu Lin, Melanie Sinclair, Carl Johnson, Sarat Pudi, Robert Bortz III, Ariel S Wirchnianski, Ethan Laudermilch, Catalina Florez, J Maximilian Fels, Cecilia M O’Brien, Barney S Graham, David Nemazee, Dennis R Burton, Ralph S Baric, James E Voss, Kartik Chandran, John M Dye, Jason S McLellan, Laura M Walker
发表日期
2020/5/16
期刊
BioRxiv
出版商
Cold Spring Harbor Laboratory Preprints
简介
Broadly protective vaccines against known and pre-emergent coronaviruses are urgently needed. Critical to their development is a deeper understanding of cross-neutralizing antibody responses induced by natural human coronavirus (HCoV) infections. Here, we mined the memory B cell repertoire of a convalescent SARS donor and identified 200 SARS-CoV-2 binding antibodies that target multiple conserved sites on the spike (S) protein. A large proportion of the antibodies display high levels of somatic hypermutation and cross-react with circulating HCoVs, suggesting recall of pre-existing memory B cells (MBCs) elicited by prior HCoV infections. Several antibodies potently cross-neutralize SARS-CoV, SARS-CoV-2, and the bat SARS-like virus WIV1 by blocking receptor attachment and inducing S1 shedding. These antibodies represent promising candidates for therapeutic intervention and reveal a new target for …
学术搜索中的文章
AZ Wec, D Wrapp, AS Herbert, D Maurer, D Haslwanter… - BioRxiv, 2020