作者
MF El‐Azab, BRB Baldowski, BA Mysona, AY Shanab, IN Mohamed, MA Abdelsaid, S Matragoon, Kathryn Elizabeth Bollinger, A Saul, AB El‐Remessy
发表日期
2014/3
期刊
British journal of pharmacology
卷号
171
期号
5
页码范围
1299-1313
简介
Background and Purpose
Retinal neurodegeneration is an early and critical event in several diseases associated with blindness. Clinically, therapies that target neurodegeneration fail. We aimed to elucidate the multiple roles by which thioredoxin‐interacting protein (TXNIP) contributes to initial and sustained retinal neurodegeneration.
Experimental Approach
Neurotoxicity was induced by intravitreal injection of NMDA into wild‐type (WT) and TXNIP‐knockout (TKO) mice. The expression of apoptotic and inflammatory markers was assessed by immunohistochemistry, elisa and Western blot. Microvascular degeneration was assessed by periodic acid‐Schiff and haematoxylin staining and retinal function by electroretinogram.
Key Results
NMDA induced early (1 day) and significant retinal PARP activation, a threefold increase in TUNEL‐positive nuclei and 40% neuronal loss in ganglion cell layer (GCL); and …
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MF El‐Azab, BRB Baldowski, BA Mysona, AY Shanab… - British journal of pharmacology, 2014