查看文章

Oxidant species is reported as a major determinant in the pathophysiology of diabetic kidney disease. However, reactive oxygen species (ROS) formation in the initial phase and progressing phase of diabetic kidney disease remains unclear. Therefore, we conducted this study to find out what ROS and their modified product are associated with eGFR in type 2 diabetes mellitus (T2DM) patients. A cross-sectional study was performed on 227 T2DM patients. The study subjects were divided into three groups based on their eGFR stage (Group 1, eGFR > 89 ml/min/1.73 m²; Group 2, eGFR = 60–89 ml/min/1.73 m²; and Group 3, eGFR < 60 ml/min/1.73 m²). Enzyme-linked immunosorbent assay (ELISA) was used to measure serum oxLDL/β₂GPI complex and urinary 8-iso-PGF2α, while ferrous ion oxidation xylenol orange method 1 (FOX-1) was used to measure urinary hydrogen peroxide (H₂O₂). H₂O₂ significantly decreased across the groups, whereas OxLDL/β₂GPI complex increased, but not significant, and there was no trend for 8-iso-PGF2α. Consistently, in the total study population, only H₂O₂ showed correlation with eGFR (r = 0.161, p = 0.015). Multiple linear regression analysis showed that significant factors for increased eGFR were H₂O₂, diastolic blood pressure, and female. Whereas increased systolic blood pressure and age were significant factors affecting the decrease of eGFR. We also found that urinary H₂O₂ had correlation with serum oxLDL/β₂GPI complex in total population. This finding could lead to further research on urinary H₂O₂ for early detection and research on novel therapies of diabetic kidney disease.