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Soils with poor engineering properties have been a concern to construction engineers because of the need to strike a balance between safety and economy during earthworks construction. This research work investigates the effects of treating a soil having poor geotechnical properties with a bio-enzyme to determine its suitability for use as road pavement layer material. The elemental composition and microstructure of the soil was determined using energy dispersive X-ray spectroscopy and scanning electron microscopy, respectively. The specific gravity, Atterberg limits, compaction, strength and permeability characteristics of the soil was determined for various dosages of the bio-enzyme. The mountain soil is classified as clayey sand and A-2–4, according to unified soil classification and AASHTO classification systems, respectively. With increasing dosage of the bio-enzyme, the plasticity index, maximum …