作者
Tamar Ben-Mordechai, Radka Holbova, Natalie Landa-Rouben, Tamar Harel-Adar, Micha S Feinberg, Ihab Abd Elrahman, Galia Blum, Fred H Epstein, Zmira Silman, Smadar Cohen, Jonathan Leor
发表日期
2013/11/12
期刊
Journal of the American College of Cardiology
卷号
62
期号
20
页码范围
1890-1901
出版商
American College of Cardiology Foundation
简介
Objectives
This study sought to investigate the hypothesis that the favorable effects of mesenchymal stromal cells (MSCs) on infarct repair are mediated by macrophages.
Background
The favorable effects of MSC therapy in myocardial infarction (MI) are complex and not fully understood.
Methods
We induced MI in mice and allocated them to bone marrow MSCs, mononuclear cells, or saline injection into the infarct, with and without early (4 h before MI) and late (3 days after MI) macrophage depletion. We then analyzed macrophage phenotype in the infarcted heart by flow cytometry and macrophage secretome in vitro. Left ventricular remodeling and global and regional function were assessed by echocardiography and speckle-tracking based strain imaging.
Results
The MSC therapy significantly increased the percentage of reparative M2 macrophages (F4/80+CD206+) in the infarcted myocardium, compared with …
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T Ben-Mordechai, R Holbova, N Landa-Rouben… - Journal of the American College of Cardiology, 2013