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The article investigates graphene metasurface based infrared biosensor. Graphene metasurface perturbations are added in the Si₃N₄ waveguide to create leaky wave structure. Biomolecules in blood plasma form are placed over the Si₃N₄ waveguide to observe the sensing characteristics. Numerical results of absorption, reflectance, electric field and sensitivity are presented in this paper. The sensitivity is calculated from the shift in the absorption peak of biosensor. The sensitivity of the proposed biosensor is also compared with the previously published biosensor designs. In addition, the design results in the form of absorption and reflectance is observed for the different physical parameters like waveguide height (S), biomolecules/air layer height(B), SiO₂ layer height(C) and graphene perturbations period(G). The corresponding electric field response is also presented for the proposed design at different …