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Murine c‐kit⁺ cardiac cells were isolated and enriched by magnetic activated cell sorting technique. c‐kit⁺ cells viability and colony‐forming activity were evaluated by MTT and clonogenic assay. c‐kit⁺ cells were exposed to endothelial, pericyte, and cardiomyocyte induction media containing 30mM glucose for 7 days. We monitored the level of endothelial (VE‐cadherin, CD31, and vWF), pericyte (NG₂, α‐SMA, and PDGFR‐β), and cardiomyocyte markers (cTnT) using flow cytometry, real‐time Polymerase Chain Reaction (PCR), and Enzyme‐Linked Immunosorbent Assay (ELISA) analyses. Ultrastructural changes were studied by transmission electron microscopy (TEM) in cells treated with 5‐Azacytidine and 30mM glucose. Matrigel plug assay was performed to determine the angio/cardiogenic property of c‐kit⁺ cells in a diabetic mouse model. Glucose of 30mM decreased c‐kit⁺ cells viability and clonogenicity (P …