作者
Shilpa Thakur, Brianna Daley, Kelli Gaskins, Vasyl V Vasko, Myriem Boufraqech, Dhaval Patel, Carole Sourbier, Jeff Reece, Sheue-Yann Cheng, Electron Kebebew, Sunita Agarwal, Joanna Klubo-Gwiezdzinska
发表日期
2018/8/15
期刊
Clinical cancer research
卷号
24
期号
16
页码范围
4030-4043
出版商
American Association for Cancer Research
简介
Purpose: Mitochondrial glycerophosphate dehydrogenase (MGPDH) is the key enzyme connecting oxidative phosphorylation (OXPHOS) and glycolysis as well as a target of the antidiabetic drug metformin in the liver. There are no data on the expression and role of MGPDH as a metformin target in cancer. In this study, we evaluated MGPDH as a potential target of metformin in thyroid cancer and investigated its contribution in thyroid cancer metabolism.
Experimental Design: We analyzed MGPDH expression in 253 thyroid cancer and normal tissues by immunostaining and examined its expression and localization in thyroid cancer–derived cell lines (FTC133, BCPAP) by confocal microscopy. The effects of metformin on MGPDH expression were determined by qRT-PCR and Western blot analysis. Seahorse analyzer was utilized to assess the effects of metformin on OXPHOS and glycolysis in …
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S Thakur, B Daley, K Gaskins, VV Vasko, M Boufraqech… - Clinical cancer research, 2018