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Purpose

To quantitatively assess the superoxide dismutase 1 (SOD1), transforming growth factor, beta 1 (TGF-β1), and dual-specificity phosphatase 1 (DUSP1) messenger ribonucleic acid (mRNA) expression levels as the main intracellular reactive oxygen species neutralizers, wound healing mediators, and immunomodulators (respectively) in keratoconic (KCN) and non-KCN corneas.

Methods

Total RNA was extracted from normal and keratoconic cultured corneal stromal fibroblasts. Semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to measure the relative expression levels of mRNAs of the SOD1, TGF-β1, and DUSP1 genes.

Results

The mRNA expression of TGF-β1 and DUSP1 was augmented in the KCN corneas (three-and fivefold, respectively; both p< 0.05). The KCN and non-KCN samples showed no difference in comparative SOD1 mRNA levels.

Conclusions

This study …