作者
Amanda Warr, Nabeel Affara, Bronwen Aken, Hamid Beiki, Derek M Bickhart, Konstantinos Billis, William Chow, Lel Eory, Heather A Finlayson, Paul Flicek, Carlos G Girón, Darren K Griffin, Richard Hall, Greg Hannum, Thibaut Hourlier, Kerstin Howe, David A Hume, Osagie Izuogu, Kristi Kim, Sergey Koren, Haibou Liu, Nancy Manchanda, Fergal J Martin, Dan J Nonneman, Rebecca E O'Connor, Adam M Phillippy, Gary A Rohrer, Benjamin D Rosen, Laurie A Rund, Carole A Sargent, Lawrence B Schook, Steven G Schroeder, Ariel S Schwartz, Ben M Skinner, Richard Talbot, Elizabeth Tseng, Christopher K Tuggle, Mick Watson, Timothy PL Smith, Alan L Archibald
发表日期
2020/6
期刊
Gigascience
卷号
9
期号
6
页码范围
giaa051
出版商
Oxford University Press
简介
Background
The domestic pig (Sus scrofa) is important both as a food source and as a biomedical model given its similarity in size, anatomy, physiology, metabolism, pathology, and pharmacology to humans. The draft reference genome (Sscrofa10.2) of a purebred Duroc female pig established using older clone-based sequencing methods was incomplete, and unresolved redundancies, short-range order and orientation errors, and associated misassembled genes limited its utility.
Results
We present 2 annotated highly contiguous chromosome-level genome assemblies created with more recent long-read technologies and a whole-genome shotgun strategy, 1 for the same Duroc female (Sscrofa11.1) and 1 for an outbred, composite-breed male (USMARCv1.0). Both assemblies are of substantially higher (>90-fold) continuity and accuracy than Sscrofa10.2 …
学术搜索中的文章
A Warr, N Affara, B Aken, H Beiki, DM Bickhart, K Billis… - Gigascience, 2020