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In this work a multidisciplinary approach for the structural assessment of masonry arch bridges is presented. The Vilanova Bridge, located in Galicia, northwest of Spain, is considered for the implementation of the methodology. A comprehensive field survey fully based on non-destructive testing techniques, which integrates laser scanning, ground penetration radar, sonic tests and ambient vibration testing, is proposed. It provides all the necessary geometric data to build an accurate and detailed three-dimensional finite element model. The calibration of the model is then carried out through the coupling with an optimization algorithm, which minimizes the discrepancies with respect to the experimentally obtained modal properties. The structural analysis of the bridge is addressed in the last stage. A sensitivity analysis involving different loading scenarios and the plausible variation of the masonry and soil material …