作者
Tiffany A Freed, Josef Coresh, Lesley A Inker, Douglas R Toal, Regis Perichon, Jingsha Chen, Kelli D Goodman, Qibo Zhang, Jessie K Conner, Deirdre M Hauser, Kate ET Vroom, Maria L Oyaski, Jacob E Wulff, Gudný Eiríksdóttir, Vilmundur Gudnason, Vicente E Torres, Lisa A Ford, Andrew S Levey
发表日期
2019/3/1
期刊
Clinical chemistry
卷号
65
期号
3
页码范围
406-418
出版商
Oxford University Press
简介
BACKGROUND
Clinical practice guidelines recommend estimation of glomerular filtration rate (eGFR) using validated equations based on serum creatinine (eGFRcr), cystatin C (eGFRcys), or both (eGFRcr-cys). However, when compared with the measured GFR (mGFR), only eGFRcr-cys meets recommended performance standards. Our goal was to develop a more accurate eGFR method using a panel of metabolites without creatinine, cystatin C, or demographic variables.
METHODS
An ultra-performance liquid chromatography–tandem mass spectrometry assay for acetylthreonine, phenylacetylglutamine, pseudouridine, and tryptophan was developed, and a 20-day, multiinstrument analytical validation was conducted. The assay was tested in 2424 participants with mGFR data from 4 independent research studies. A new GFR equation (eGFRmet) was developed in a …
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TA Freed, J Coresh, LA Inker, DR Toal, R Perichon… - Clinical chemistry, 2019