作者
Esther N Klein Hesselink, Carles Zafon, Nuria Villalmanzo, Carmela Iglesias, Bettien M van Hemel, Mariëlle S Klein Hesselink, Cristina Montero-Conde, Raquel Buj, Dídac Mauricio, Miguel A Peinado, Manel Puig-Domingo, Garcilaso Riesco-Eizaguirre, Jordi L Reverter, Mercedes Robledo, Thera P Links, Mireia Jorda
发表日期
2018/2
期刊
The Journal of Clinical Endocrinology & Metabolism
卷号
103
期号
2
页码范围
397-406
出版商
Endocrine Society
简介
Context
Global DNA hypomethylation is a major event for the development and progression of cancer, although the significance in thyroid cancer remains unclear. Therefore, we aimed to investigate its role in thyroid cancer progression and its potential as a prognostic marker.
Methods
Global hypomethylation of Alu repeats was used as a surrogate marker for DNA global hypomethylation, and was assessed using the Quantification of Unmethylated Alu technique. Mutations in BRAF and RAS were determined by Sanger sequencing.
Results
Ninety primary thyroid tumors were included [28 low-risk differentiated thyroid cancer (DTC), 13 pediatric DTC, 33 distant metastatic DTC, 7 poorly differentiated thyroid cancer (PDTC), and 9 anaplastic thyroid cancer (ATC)], as well as 24 distant metastases and 20 normal thyroid tissues. An increasing hypomethylation …
学术搜索中的文章
EN Klein Hesselink, C Zafon, N Villalmanzo, C Iglesias… - The Journal of Clinical Endocrinology & Metabolism, 2018