作者
JJH Eijsink, N Yang, A Lendvai, HG Klip, HH Volders, HJ Buikema, BM van Hemel, M Voll, HJT Coelingh Bennink, E Schuuring, GBA Wisman, AGJ van der Zee
发表日期
2011/2/1
期刊
Gynecologic Oncology
卷号
120
期号
2
页码范围
280-283
出版商
Academic Press
简介
OBJECTIVE
To explore the feasibility of DNA methylation analysis for the detection of cervical neoplasia in self-obtained cervico-vaginal lavages.
METHODS
Lavages collected by a self-sampling device and paired cervical scrapings were obtained from 20 cervical cancer patients and 23 patients referred with an abnormal cervical smear (15 with high-grade cervical intraepithelial neoplasia (CIN2+) and 8 without CIN). All lavages and scrapings were analyzed by liquid based cytology (LBC), Hybrid Capture II (HC-II) for hr-HPV DNA detection and by DNA methylation analysis (JAM3, TERT, EPB41L3 and C13ORF18). Concordance between lavages and scrapings was measured by Cohen's Kappa (k).
RESULTS
In lavages and scrapings from cervical cancer patients (n=20), methylation analysis was positive in 19 (95%) and 19 (95%), HC-II in 16 (80%) and 15 (75%) and LBC in 15 (75%) and 19 (95%), respectively …
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JJH Eijsink, N Yang, A Lendvai, HG Klip, HH Volders… - Gynecologic Oncology, 2011