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The research paper proposes a novel algorithm for DNA steganography, a technique that involves hiding information within DNA sequences. Our algorithm is based on amino acids and one of its property called ambiguity which uses RNA codons to secure the first layer of encoding. Cryptographic algorithms are used to make more secure for second layer of encryption. In this research paper, we will describe the proposed algorithm in detail, including its encoding and decoding procedures. We will also present experimental results demonstrating the feasibility and effectiveness of our approach. The results obtained from the experimental analysis demonstrate the efficiency of the algorithm provides low cracking probability, making it a promising solution for secure communication applications.