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This paper presents a specifically-built novel all four limbs-based human energy harvester with bio-feedback and criteria to maximize human bio-mechanical energy output at a defined power level. The developed energy harvester decouples the human upper and lower limbs energy generation while giving a full-body workout. A Graphical User Interface was embedded to monitor the human rider's heart rate, respiratory rate, generated electrical energy, lower and upper limbs power in real-time. In the implemented energy maximization criteria, an optimum leg-to-hand power ratio was obtained. This optimum power ratio maximizes the rider's energy output at a defined power level. The output energy variations of untrained Sri Lankan citizens were acquired experimentally using pre-defined power levels and the maximum and minimum energy output were observed at 60W and 120W power levels for each test subject …