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Wool fibers were dyed with natural dye extracted from Madder plant using conventional and microwave heating technique. Wool fibers pretreated with chitosan and tannic acid. The dyeing properties were affected with some factors such as dye extraction, dye concentrations, pH values and the time of dyeing. Color strength was measured for dyed wool fibers. The fastness properties including washing, rubbing, perspiration and light of dyed fibers were measured. The color strength of dyed wool fibers and dye extraction indicates that microwave heating is more effective method than conventional heating. The treated samples exhibited higher results than the untreated. The results illustrated that the pretreatment by chitosan dissolved in citric acid is higher than chitosan in acetic acid compared to untreated dyed wool fibers Dyes Microwaves technique are economic, saving time of dyeing and energy, eco-friendly and enhance the dyeing properties. The results show also good fastness properties. It is necessary to promote non-polluting natural dyes, which involve inexpensive equipment and small scale operations.