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The main objective of the study was the identification of antibacterial activity of lemon (Citrus limon L.) peel essential oil and argan (Argania spinosa L.) oil against fish pathogenic bacteria. Antibacterial activity was determined against six different fish pathogens (Yersinia ruckeri, Aeromonas hydrophila, Listonella anguillarum, Edwarsiella tarda, Citrobacter freundii and Lactococcus garvieae). Essential oil derived from lemon peel and argan oil were applied against the bacteria using the disc diffusion and micro dilution method under in vitro conditions. The disc diffusion results indicated that essential oil of naturally C. limon peel and argan oil significantly inhibited the growth of Y. ruckeri, A. hydrophila, L. anguillarum and C. freundii. Our results suggested that the use of lemon peel essential oil and argan oil induced a stronger antibacterial effect.