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The maritime transportation flows and container demand have been increasing over time, although the COVID-19 pandemic may slow down this trend for some time. One of the common strategies adopted by shipping lines to efficiently serve the existing customers is the deployment of large ships. The current practice in the liner shipping industry is to deploy a combination of ships of different types with different carrying capacities (i.e., heterogeneous fleet), especially at the routes with a significant demand. However, heterogeneous fleets of ships have been investigated by a very few studies addressing the tactical liner shipping decisions (i.e., determination of service frequency, ship fleet deployment, optimization of ship sailing speed, and design of ship schedules). Moreover, limited research efforts have been carried out to simultaneously capture all the major tactical liner shipping decisions using a single solution …