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This paper addresses L₂ observer-based fault detection issues for a class of nonlinear systems in the presence of parametric and dynamic uncertainties, respectively. To this end, three different types of uncertain affine nonlinear system models studied in this paper are described first. Then, the integrated design schemes of L₂ observer-based fault detection systems are derived with the aid of Hamilton–Jacobi inequalities (HJIs), respectively. Numerical examples are also provided in the end to demonstrate the effectiveness of the proposed results.