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Inorganic polyphosphates (polyPs) are linear polymers of orthophosphate residues linked by phosphoanhydride bonds similar to ATP. PolyPs are ubiquitously distributed in nature from bacteria to mammalian cells where they play multiple roles. Here we investigated intracellular localization of polyP in cancer cell lines and tested the idea about the involvement of polyP in cell growth.

To study PolyP localization and cellular levels we used DAPI-based method of polyP staining. Experiments were performed using the following cell lines: H1299 (lung cancer), U251 (glioma) and HEK293 as a control cell line. PolyP was also visualized by immunocytochemistry with X-press tagged PolyP-binding domain of exopolyphosphatase (PPX) and subsequent use of specific antibodies. The role of PolyP in cancer cell proliferation was studied using MTS proliferation assay. In these experiments endogenous PolyP was either …