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Objectives: Sarcoidosis is an inflammatory disease characterized by granuloma tissue lesions that most commonly affect the lungs, but can target any other organ. While the cause of sarcoidosis and the mechanisms underlying granuloma formation and progression are still unknown, the disease shares many similarities with tuberculosis, including histological characteristics, organ tropism and clinical presentation. We hypothesized that circulating monocytes from patients with sarcoidosis or tuberculosis may retain a specific epigenetic signature, distinguishing from healthy controls and each disease. Methods: To test this, we performed genome-wide epigenetic profiling of circulating monocytes from newly diagnosed patients with tuberculosis or sarcoidosis using the CUT&Tag technology, and assessed the acetylation of lysine 27 of histone 3 (H3K27Ac)(the most appropriate histone mark to identify enhancers of …