作者
Szabolcs Fekete, Jean-Luc Veuthey, Davy Guillarme
发表日期
2012/10/1
来源
Journal of pharmaceutical and biomedical analysis
卷号
69
页码范围
9-27
出版商
Elsevier
简介
In the pharmaceutical field, there is considerable interest in the use of peptides and proteins for therapeutic purposes. There are various ways to characterize such complex samples, but during the last few years, a significant number of technological developments have been brought to the field of RPLC and RPLC–MS. Thus, the present review focuses first on the basics of RPLC for peptides and proteins, including the inherent problems, some possible solutions and some directions for developing a new RPLC method that is dedicated to biomolecules. Then the latest advances in RPLC, such as wide-pore core–shell particles, fully porous sub-2μm particles, organic monoliths, porous layer open tubular columns and elevated temperature, are described and critically discussed in terms of both kinetic efficiency and selectivity. Numerous applications with real samples are presented that confirm the relevance of these …
学术搜索中的文章
S Fekete, JL Veuthey, D Guillarme - Journal of pharmaceutical and biomedical analysis, 2012