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A smart illumination system (ILSys) to study the growth process of green plants was developed. This system can control commercial LED lamps that can be turned on to emit continuous or pulsed light. ILSys was applied to determine the effect of pulsed light on the fluorescence emission in tomato plants (Lycopersicon esculentum). The fluorescence emission was measured when the plants were radiated with continuous light and light pulsed at different frequencies (0.1Hz, 1Hz, 10Hz, 50Hz, 100Hz, 500Hz, 1kHz, 5kHz, 10kHz, 50kHz and 100kHz). For the experiment three red and blue-LEDs lamps and a Pulse Modulated Chlorophyll Fluorescence Monitoring System were connected to the ILSys. Results showed the highest quantum efficiencies in the Photosystem II (φPSII) was obtained when applying pulse frequency of 0.1Hz and 1Hz with 50% light pulse width. ILSys allowed the finding of a significant difference in …