作者
C Dunsby, PMP Lanigan, J McGinty, DS Elson, J Requejo-Isidro, I Munro, N Galletly, F McCann, B Treanor, Björn Önfelt, DM Davis, MAA Neil, PMW French
发表日期
2004/11/19
期刊
Journal of Physics D: Applied Physics
卷号
37
期号
23
页码范围
3296
出版商
IOP Publishing
简介
Fluorescence imaging is used widely in microscopy and macroscopic imaging applications for fields ranging from biomedicine to materials science. A critical component for any fluorescence imaging system is the excitation source. Traditionally, wide-field systems use filtered thermal or arc-generated white light sources, while point scanning confocal microscope systems require spatially coherent (point-like) laser sources. Unfortunately, the limited range of visible wavelengths available from conventional laser sources constrains the design and usefulness of fluorescent probes in confocal microscopy. A'hands-off'laser-like source, electronically tunable across the visible spectrum, would be invaluable for fluorescence imaging and provide new opportunities, eg automated excitation fingerprinting and in situ measurement of excitation cross-sections. Yet more information can be obtained using fluorescence lifetime …
引用总数
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