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The development of stabilized silver nanoparticles (AgNP) is paramount in preventing the build-up of pathogenic biofilms. AgNP also requires a suitable support system to deliver the AgNP directly to the bacteria. Herein, AgNP-supported zeolite A was prepared via the in situ phytosynthesis method using Orthosiphon aristatus extract based on our previous work. The optimal condition of AgNP biosynthesis was achieved at 0.4 mL of the leaf extract in 10 mL AgNO₃ 1 mM. The AgNP was incorporated into zeolite A using the in situ approach. The developed materials were characterized by their porosity, surface area, thermogravimetry, and zeta potential. The morphology of samples was distinguished using field emission scanning electron microscopy (FESEM). The distribution of AgNP into the macroporous zeolite A was measured according to the reduction of specific pore volume from 0.1033 to 0.0346 cm³/g. Anti …