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The capability of synthesized zeolite A (SZ) to immobilize Ag ions (Ag-SZ) and Ag nanoparticles (AgNp-SZ) were comparatively studied. A novel approach of in situ biosynthesized AgNP-incorporated synthesized zeolite A (AgNp-SZ) was synthesized at an optimum volume of 0.4 mL of the Orthosiphon aristatus (O. aristatus) leaves plant extract (5%) using an in situ approach. In comparison, Ag-SZ was produced by loading the synthesized zeolite with Ag ions. All synthesized materials were characterized for their morphologies and physicochemical properties. The characterization analyses validate that the biosynthesized AgNP (<100 nm) using O. aristatus leaves extract was incorporated into the zeolite A. The antibacterial testing confirmed that these materials have antibacterial activity against Escherichia coli ATCC 11229 and Staphylococcus aureus ATCC 6538. MIC/MBC analysis demonstrated that in 0.9 …