作者
Yangbo Hu, Pei Lu, Yong Zhang, Yunlong Li, Lamei Li, Li Huang, Shiyun Chen
发表日期
2011/4/1
期刊
FEMS microbiology letters
卷号
317
期号
2
页码范围
190-195
出版商
Blackwell Publishing Ltd
简介
Survival in acidic environments is important for successful infection of gastrointestinal pathogens. Many bacteria have evolved elaborate mechanisms by inducing or repressing gene expression, which subsequently provide pH homeostasis and enable acid survival. In this study, we employed comparative proteomic analysis to identify the acid-responsive proteins of a food-borne enteric bacterium, Yersinia pseudotuberculosis. The expression level of eight proteins involved in carbohydrate metabolism was up- or downregulated over twofold at pH 4.5 compared with pH 7.0. The role of a global transcriptional regulator catabolite repressor/activator Cra was further studied in this acid survival process. lacZ-fusion analysis showed that expression of cra was repressed under acidic pH. Deletion of the cra gene increased acid survival by 10-fold, whereas complementation restored the wild-type phenotype. These …
学术搜索中的文章
Y Hu, P Lu, Y Zhang, Y Li, L Li, L Huang, S Chen - FEMS microbiology letters, 2011