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Objective To study association between nocturnal mobile phone use and mental health, suicidal feelings, and self-injury in adolescents. Methods Associations of mobile phone use after lights out with mental health, suicidal feelings, and self-injury were cross-sectionally examined in 17,920 adolescents using a self-report questionnaire. A series of logistic regression analyses were separately conducted for early (grades 7–9) and late (grades 10–12) adolescents. Results Sleep length was significantly associated with the mobile phone use only in early adolescents. Logistic regression showed significant associations of the nocturnal mobile phone use with poor mental health, suicidal feelings, and self-injury after controlling for sleep length and other confounders. Conclusions Mobile phone use after lights out may be associated with poor mental health, suicidal feelings, and self-injury in both early …