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Abstract Martensitic Ti6Al4V samples were fabricated by laser powder bed fusion additive manufacturing, water quenching of wrought and the additively manufactured Ti6Al4V samples respectively, their microstructural characteristics, tensile properties and deformation behaviours have been investigated in this study. These samples showed differences in grain orientations, size of the prior β grains, thickness of the martensite (α′) laths, the lattice strain and presence of the β phase between the α′ laths. A relationship between the yield strength and the thickness of α′ laths and lattice strain has been developed. The significant difference in the ductility in terms of the total strain at fracture among the martensitic Ti6Al4V samples processed by the different routes has been observed and investigated. The additively manufactured Ti6Al4V showed the highest post-uniform plastic deformation (deformation after necking …