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An in vitro method was developed for the production of phenolic compounds from callus suspension cultures of Habenaria edgeworthii Hook. f. ex. Collett, a rare Himalayan medicinal orchid. Friable and light yellow coloured callus was induced within eight weeks when seeds were cultured on Murashige and Skoog (MS) or ½ MS medium supplemented with 1μM α-naphthaleneacetic acid (NAA). In order to increase the biomass, following another 2 weeks these calli were repeatedly subcultured (4 week interval) onto ½ MS liquid medium supplemented with 0.1μM 6-benzyladenine (BA). The effect of BA (0.0–3.0μM) and methyl jasmonate (MeJA; 10–1000μM) treatment on callus growth, total phenolic content and corresponding antioxidant activity was also determined following 4 weeks of culture on ½ MS medium (liquid). Maximum callus biomass (2.61g fresh weight) was obtained in medium containing 1.0μM BA …