查看文章

Neurotransmitter‐related messenger RNAs were detected by in situ hybridization in sections of rat and mouse brains by using ³⁵S‐radiolabelled RNA probes transcribed from cDNAs cloned in SP6 promoter‐containing vectors. The distribution of messenger RNAs for glutamic acid decarboxylase, tachykinins (substance P and K), and tyrosine hydroxylase was examined in the striatum, pallidum, and substantia nigra. Dense clusters of silver grains were observed with the RNA probe complementary of the cellular messenger RNA for glutamic acid decarboxylase (antisense RNA) over most large neurons in the substantia nigra pars reticulata and medium‐sized to large neurons in all pallidal subdivisions. A few very densely and numerous lightly labelled medium‐sized neurons were present in the striatum. Among the areas examined, only the striatum contained neurons labelled with the antisense tachykinin RNA …