作者
Anna Baumgarten, Claudia Bang, Anika Tschirner, Anke Engelmann, Volker Adams, Stephan Von Haehling, Wolfram Doehner, Reinhard Pregla, Markus S Anker, Kinga Blecharz, Rudolf Meyer, Roland Hetzer, Stefan D Anker, Thomas Thum, Jochen Springer
发表日期
2013/9/30
期刊
International journal of cardiology
卷号
168
期号
2
页码范围
1447-1452
出版商
Elsevier
简介
Background
The transcription factor TWIST1 has been described to regulate the microRNA (miR)-199/214 cluster. Genetic disruption of TWIST1 resulted in a cachectic phenotype and early death of the knock-out mice. This might be connected to the activity of the ubiquitin-proteasome-system (UPS), as miR-199a has been suggested to regulate the ubiquitin E2 ligases Ube2i and Ube2g1.
Methods
Cardiac tissue from explanted hearts of 42 patients with dilated cardiomyopathy and 20 healthy donor hearts were analysed for protein expression of TWIST1 and its inhibitors Id-1, MuRF-1 and MAFbx, the expression of miR-199a, -199b and -214, as well as the activity of the UPS by using specific fluorogenic substrates.
Results
TWIST1 was repressed in patients with dilated cardiomyopathy by 43% (p = 0.003), while Id1 expression was unchanged. This was paralleled by a reduced expression of miR-199a by 38 ± 9% (p = 0 …
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